A Stress Response ASMS 2010 Comparison: LC/MS
dry sample was wet milled using a Retsch MM301 ball mill mixer,in 2 mL Eppendorf tubes in an extraction solvent of 6:3:3 chloroform:methanol:water with a single 5 mm ball bearing used as a tube insert to facilitate mechanical rupture of the yeast. External standards were added prior to milling. Nine samples for each condition were processed
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Retsch - operating manuals - operating instructions
On this page you will find PDF downloads of RETSCH operating instructions. Operation manuals for all active mills and sieve shakers are available.
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Mills - PoC RT-PCR Covid-19 Test Gerät
Retsch PM100 Planetary Ball Mill Planeten-Kugelmühle Dry/Wet (Year 2015) ... Retsch MM301. Retsch MM301. New cover Drive: single phase AC motor Vibration frequency of the grinding jar fastening Rated power approx 184 VA IP …
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In Vitro and In Vivo Evaluation of Amorphous Solid ...
For pulverization and milling, a Retsch MM301 ball mill system (Haan, Germany) was used. Typically, sample was milled with a frequency of 20 1/s and duration of 5 min. Fast Evaporation Solid Dispersion (Evap ASD) of Griseofulvin (20% Drug Load)
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Physical and Genetic Interactions Between Uls1 and the ...
The resuspended culture was frozen in liquid nitrogen, collected into a pre-chilled 50-ml tube, and lysed with a Retsch MM301 ball mill. RT PCR assays To isolate RNA, 10 ml of cell culture (1 × 10 7 cells/ml) was centrifuged, washed with 1 ml of H 2 O, and resuspended in 0.2 ml of RNA breaking buffer (0.5 M NaCl, 0.2 M Tris-HCl [pH 7.6], 0.01 ...
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Used Planetary Ball Mill for sale. Retsch equipment & more ...
Retsch PM400 Planetary Ball Mill (2014) Manufacturer: Retsch Model: PM400 EquipX inc Manufacture date 2014 Also available: 2 stainless grinding jars 500ml (2) Retsch pn. : 01 462 0228 Stainless steel Grinding Jars with lid, 500 mL Price $1950. each Retsch...
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RETSCH –,
RETSCH,、。 ... TM 500: Our ball mill for large sample volumes - 316 L-Version available ; : ! > ...
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UC Davis - escholarship
(4) Ball mill/ultrasonication: The sample was ground with a Retsch MM301 Ball Mill at 1800 rpm and then ultrasonicated for ten minutes. (5) Homogenizer: An IKA Ultra turrax T10 Basic disperser was used until the tissue was homogenous. This technique was repeated twice (trial 1 and trial 2).
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Folate Polyglutamylation is Required for Rice Seed ...
Folates extraction from rice leaf and grain samples. Three replicates of rice grain (0.5 g) and of leaf samples (0.2 g) were homogenised using Retsch® MM301 ball mill equipment and mixed with 1 ml ice-cold 95% methanol/phosphate extraction buffer (75 mM KH2PO4, 0.4 M ascorbic acid, 0.8% 2-mercaptoethanol, pH 6.0) and 25 µl internal standard
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Planetary Ball Mill Range - RETSCH - Introduction - YouTube
A Planetary Ball Mill is used wherever the highest degree of fineness is required. The RETSCH laboratory ball mill range meets all requirements for fast and ...
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Ball Mill - RETSCH - powerful grinding and homogenization
RETSCH is the world leading manufacturer of laboratory ball mills and offers the perfect product for each application. The High Energy Ball Mill E max and MM 500 were developed for grinding with the highest energy input. The innovative design of both, the mills and the grinding jars, allows for continuous grinding down to the nano range in the shortest amount of time - with …
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Retsch : Retsch : Do pobrania : Video
Informujemy, iż w celu optymalizacji treści dostępnych w naszym serwisie oraz dostosowania ich do Państwa indywidualnych potrzeb korzystamy z informacji zapich za pomocą plików cookies na urządzeniach końcowych użytkowników.
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Retsch | Glen Mills, Inc.
The robust high-performance drive makes the mill suitable for long-term grinding processes up to 99 hours. Hence, the MM 500 is the first mixer mill in the market to provide a real alternative to grinding in a planetary ball mill – with more comfortable handling and less warming effects.
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Improved HPLC method for rooibos phenolics targeting ...
Prior to extraction the milled plant material was further refined using a Retsch MM301 ball mill to improve sample homogeneity for mini-scale extraction. The finely milled rooibos plant material samples were subjected to extraction as described by Joubert et al. (2014) .
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Compound Identifi cation, Profi ling and Pathway Analysis of ...
Retsch MM301 ball mill mixer was used with a single 5-mm ball bearing as a tube insert to facilitate mechanical rupture of the yeast. External standards were added prior to milling. Nine replicate samples for each culture condition were processed for 3 × 1 minute cycles at 30 Hz. The milling process resulted in polar and nonpolar solvent phases.
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Size reduction with Planetary Ball Mills - retsch.dk
RETSCH Planetary Ball Mills are used for the pulverization of soft, fibrous, hard and brittle ma-terials. They achieve a very high final fineness down to the submicron range. The feed size depends on the mill and can be as large as 10 mm. If the …
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Characterization and combustion of olive pomace and forest ...
Therefore, a Retsch MM301 ball mill was applied during 5 min at 25 Hz, as well as a sieve of the corresponding size. 2.3. Densification and categorization of the samples. As soon as the samples were prepared, the pelletizing process was carried out by means of a small GR150E2 pelletizer.
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The use of an acetoacetyl‐CoA synthase in place of a β ...
The lyophilized leaf material was ground to a fine powder in a Retsch MM301 ball mill. Finely ground dried biomass was extracted with CD 2 Cl 2 (~ 9 g, 44 h stirring at RT), filtered (0.22‐μm syringe filter) and the organic filtrate solution analysed by 1 H NMR (400 MHz, Spectral Data Services, Inc., Champaign, IL, USA).
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Quantification of Process Induced Disorder in Milled ...
An oscillatory ball mill (Mixer Mill MM301, Retsch & Co., Germany) was used for cryo milling as follows: 1 g of indomethacin was placed into a 25 mL milling jar and milled with two 12 mm balls for 120 minutes. The jars were immersed in liquid nitrogen for 3 min before milling and repeatedly for 3 min at 15 min intervals.
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Mixer Mill MM 301 ( Retsch ) | EVISA's Instruments ...
Mixer Mill MM 301. Retsch mixer mills MM 301 are particularly suited for dry and wet grinding of small quantities of material. The mills are ideal for mixing and …
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A bacterial-like mechanism for transcription termination ...
The yeast Sen1p helicase is involved in the transcription termination of most noncoding RNAs transcribed by RNA polymerase II. In vitro analyses now show that Sen1p is the key enzyme in the ...
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Planetary Ball Mill PM 400 - RETSCH - powerful and quick ...
Planetary Ball Mills are used wherever the highest degree of fineness is required.Apart from the classical mixing and size reduction processes, the mills also meet all the technical requirements for colloidal grinding and have the energy input necessary for mechanical alloying processes.The extremely high centrifugal forces of planetary ball mills result in very high pulverization energy …
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Quick n' Cheap – a simplified workflow to barcode ...
adaptor for a Retsch MM301 ball mill. To break the spores, the rack was shaken at 30 Hz for 70 seconds. If no mill is available, the rack can as well be vor-texed 3 …
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Isolation and purification of ent -pimara-8(14),15-diene ...
Extraction conditions of the sonication method have been described earlier. 20 Filtered and freeze-dried A. nidulans mycelia were homogenized with a Retsch MM301 Ball Mill at 29 Hz for 30–60 s. The homogenized powder was mixed with diatomaceous earth in ASE sample cells (Dionex) and extracted with ASE 200 (Dionex) using hexane : ethyl acetate ...
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KDM5 lysine demethylases are involved in maintenance of 3 ...
Cells were then frozen in liquid nitrogen, cryogenically lysed using a Retsch MM301 ball mill, and thawed in immunoprecipitation (IP) buffer [20 mM Hepes-NaOH (pH 7.4), 2 mM MgCl 2, 300 mM NaCl, 0.1% Tween 20, and 1 / 100 protease inhibitor cocktail]. Deoxyribonuclease I (DNase I) was added to each lysate at 2% (w/v).
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Mixer Mill MM 400 - RETSCH - powerful grinding by …
The Mixer Mill MM 400 is a compact, versatile bench-top unit developed specially for dry, wet and cryogenic grinding of small sample amounts. This laboratory mill mixes and homogenizes up to 2 x 20 ml powders and suspensions within a few seconds.
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Retsch, Inc. - Fisher Sci
RETSCH is active in the fields of homogenizing laboratory samples for analysis as well as particle size analysis of solid substances. 1 – 30 of Products Industries
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